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KMID : 1145120170150030281
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Asian Journal of Beauty and Cosmetology
2017 Volume.15 No. 3 p.281 ~ p.290
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Efficacy of a Cosmetic Material from Complex Extracts of Vaccinium spp., Phellinus linteus, Castanea crenata, and Cimicifuga heracleifolia
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Jang Young-Ah
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Abstract
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Purpose: To confirm the possibility of using complex extracts of Vaccinium spp., Phellinus linteus, Castanea crenata, and Cimicifuga heracleifolia for cosmetic substances, we tested anti-oxidant and anti-inflammatory activities.
Methods: The complex extracts of Vaccinium spp., Phellinus linteus, Castanea crenata, and Cimicifuga heracleifolia were used in a ratio of 1:1:1:1, and these complexes were extracted in 70% ethanol. To evaluate the anti-oxidant activity, the complexes were concentrated and the scavenging activities of the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) cation were assessed. The anti-inflammatory activity was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, western blot analysis, and real-time polymerase chain reaction (real-time PCR).
Results: Complex extracts showed 83.1% DPPH and 98.4% ABTS+ radical scavenging activities at a concentration of 1000 μg/mL. The MTT assay revealed cell viability over 81.0% at 10, 25, and 50 μg/mL concentrations of the complex extracts and cell death over 21.0% at 100 and 250 μg/mL concentrations of the complex extracts. Therefore, the experimental concentration of the complex extracts was 10?50 μg/mL. Western blot analysis revealed that at 50 μg/mL concentration of the complex extracts, the expression level of nitric oxide (NO) was decreased by 28.0% and those of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2) were decreased by 32.8% and 48.0%, respectively. Real-time PCR showed a decrease in iNOS and COX2 mRNA expression levels in a concentration-dependent manner.
Conclusion: Complex extracts have excellent anti-oxidant and anti-inflammatory effects, and we expect that these extracts will serve as functional cosmeceutical materials.
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KEYWORD
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Complex extracts, Anti-oxidant effects, Anti-inflammatory effects, Functionality, Cosmeceutical
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